Sterile Cell Culture Flask for Cell Growth and Expansion

Cell culture flask suitable for long-term cell culture, passage, and expansion of cells, it is a commonly used basic culture consumable in life science laboratories. The product features a relatively closed bottle body design with a small bottle opening to help reduce the risk of contamination; compatible with sealing caps or membrane caps to meet usage requirements under different culture conditions.

Transparent cell culture flask with blue-green vented cap.

Structure

The cell culture flask primarily consists of the bottle body, neck, cap, culture surface, and labeling area.

Bottle Body: Manufactured from transparent polystyrene material to facilitate observation of cell growth status.
Bottle Neck: Designed with a slanted neck to facilitate pouring of culture medium and to allow easy access for pipettes and cell scrapers.
Bottle Cap: Made of high-density polyethylene, with options for a sealing cap or membrane cap according to experimental requirements.
Filter Membrane: Membrane material can be PVDF/PTFE, featuring excellent gas permeability, suitable for culture environments requiring continuous gas exchange.
Marking Area: Both sides of the flask body have volume graduations, with a frosted writing area near the neck for easy observation and labeling.

Cell Culture Flask Structure

Product Details

CAPACITY RANGE

25–850 mL

Untreated and TC-treated surfaces

Sealing and membrane cap options

Specification

Capacity:25–850 mL

Maximum Working Volume:8–200 mL

Sterilization Method:Radiation sterilization

Cap Types:Sealing cap, membrane cap

Surface:Untreated surface, TC-treated surface

Model:T12.5, T25, T75, T150, T175, T182, T182 (Tall), T225, T300

Table 1: Cell Culture Flask (Untreated) Parameters

No.	Capacity (mL)	Cell Growth Surface Area (cm²)	Maximum Working Volume (mL)	Cap Type	Length (mm)	Width (mm)	Height (mm)	Inner diameter (mm)	pcs/bag	pcs/box
LCF01025	25	12.5	8	Sealing Cap	73.7	40.4	22.8	14.2	10	200
LCF02025	25	12.5	8	Membrane Cap	73.7	40.4	22.8	14.2	10	200
LCF01050	50	25	17.5	Sealing Cap	92.9	49.5	29.1	18.2	10	200
LCF02050	50	25	17.5	Membrane Cap	92.9	49.5	29.1	18.2	10	200
LCF01250	250	75	60	Sealing Cap	152.5	81.5	35.2	25	5	100
LCF02250	250	75	60	Membrane Cap	152.5	81.5	35.2	25	5	100
LCF01375	375	150.0 (U Shape)	140	Sealing Cap	199.7	111.3	111.3	25.6	5	50
LCF02375	375	150.0 (U Shape)	140	Membrane Cap	199.7	111.3	111.3	25.6	5	50
LCF01600	600	175	250	Sealing Cap	199.9	112.7	49.3	25.7	5	50
LCF02600	600	175	250	Membrane Cap	199.9	112.7	49.3	25.7	5	50
LCF01182	600	182	125	Sealing Cap	219.3	115.7	38.3	29.5	5	40
LCF02182	600	182	125	Membrane Cap	219.3	115.7	38.3	29.5	5	40
LCF01200	600	182.0 (Extended-height)	200	Sealing Cap	219.3	115.7	49.5	29.5	5	40
LCF02200	600	182.0 (Extended-height)	200	Membrane Cap	219.3	115.7	49.5	29.5	5	40
LCF01850	850	225	400	Sealing Cap	221.9	137.2	49.5	25.7	5	25
LCF02850	850	225	400	Membrane Cap	221.9	137.2	49.5	25.7	5	25
LCF01300	850	300	200	Sealing Cap	269.2	166	47	29.5	3	18
LCF02300	850	300	200	Membrane Cap	269.2	166	47	29.5	3	18

Table 2: Cell Culture Flask (TC Treated) Parameter

No.	Capacity (mL)	Cell Growth Surface Area (cm²)	Maximum Working Volume (mL)	Recommended Working Volume (mL)	Cap Type	Length (mm)	Width (mm)	Height (mm)	Inner diameter (mm)	pcs/bag	pcs/box
LTCF11025	25	12.5	8	2.5–3.75	Sealing Cap	73.7	40.4	22.8	14.2	10	200
LTCF12025	25	12.5	8	2.5–3.75	Membrane Cap	73.7	40.4	22.8	14.2	10	200
LTCF11050	50	25	17.5	5–7.5	Sealing Cap	92.9	49.5	29.1	18.2	10	200
LTCF12050	50	25	17.5	5–7.5	Membrane Cap	92.9	49.5	29.1	18.2	10	200
LTCF11250	250	75	60	15–22.5	Sealing Cap	152.5	81.5	35.2	25	5	100
LTCF12250	250	75	60	15–22.5	Membrane Cap	152.5	81.5	35.2	25	5	100
LTCF11375	375	150.0 (U Shape)	140	30–45	Sealing Cap	199.7	111.3	111.3	25.6	5	50
LTCF12375	375	150.0 (U Shape)	140	30–45	Membrane Cap	199.7	111.3	111.3	25.6	5	50
LTCF11600	600	175	250	35–52.5	Sealing Cap	199.9	112.7	49.3	25.7	5	50
LTCF12600	600	175	250	35–52.5	Membrane Cap	199.9	112.7	49.3	25.7	5	50
LTCF11182	600	182	125	36.4–54.6	Sealing Cap	219.3	115.7	38.3	29.5	5	40
LTCF12182	600	182	125	36.4–54.6	Membrane Cap	219.3	115.7	38.3	29.5	5	40
LTCF11200	600	182.0 (Extended-height)	200	36.4–54.6	Sealing Cap	219.3	115.7	49.5	29.5	5	40
LTCF12200	600	182.0 (Extended-height)	200	36.4–54.6	Membrane Cap	219.3	115.7	49.5	29.5	5	40
LTCF11850	850	225	400	45–67.5	Sealing Cap	221.9	137.2	49.5	25.7	5	25
LTCF12850	850	225	400	45–67.5	Membrane Cap	221.9	137.2	49.5	25.7	5	25
LTCF11300	850	300	200	60–90	Sealing Cap	269.2	166	47	29.5	3	18
LTCF12300	850	300	200	60–90	Membrane Cap	269.2	166	47	29.5	3	18

Features

Convenient screw cap operation. The bottle cap rotates about 1/4 turn for easy opening and closing, reducing repetitive steps.
Stable stacking. Designed for stable stacking, facilitating placement, transfer, and batch management inside the incubator.
Clean and sterile assurance. Radiation sterilized to achieve SAL 10⁻⁶ sterility level, free of DNase/RNase, endotoxins, and cytotoxicity.
Consistent batch quality. Product specifications, surface treatment, and cleanliness indicators remain stable, making it suitable for long-term repurchase and bulk procurement.
Packaging options available. Supports single and bulk packaging, convenient for scientific research, biopharmaceuticals, quality control laboratories, and distribution.

Application

Cell culture flask suits routine cell culture, passage maintenance, and cell expansion. Different specifications, surface treatments, and cap configurations can be selected according to cell type, culture scale, and aeration requirements.

Routine cell culture: Suitable for routine culture of adherent cells and some suspension cells.
Cell passage and expansion: Used for cell line maintenance, continuous passage, and pre-experiment cell expansion.
Subsequent experiment preparation: Provide a stable cell source for transfection, detection, pharmacodynamic evaluation, and other experiments.

Transparent cell culture flask with blue breathable cap, stacked inside the incubator.

Cell culture flasks cultured in bulk inside the incubator